Cloning and characterization of human tumor cell interstitial collagenase.

Gelatinase Expression of Interstitial Collagenase, Stromelysin, and 72-kDa Tumor Cell-derived Collagenase-stimulatory Factor Increases

The tumor cell-derived collagenase-stimulatory factor (TCSF) was pre viously purified from human lung carcinoma cells (S. M. Ellis, K. Nabeshima, and C. Biswas, Cancer Res., 49: 3385-3391, 1989). This protein is present on the surface of several types of human tumor cells in vitro and in vivo and stimulates production of interstitial collagenase in human fibroblasts. In this study it is shown t...

Collagenase Cloning and Characterization of Human Tumor Cell Interstitial

A full-length complementary DNA (cDNA) for interstitial collagenase was isolated from an A2058 melanoma cDNA library using the pCD-X Okayama-Berg vector. The tumor interstitial collagenase cDNA was sequent-eeland compared to the published sequences for human fibroblast collagenase. The sequence for the tumor collagenase has two DNA base pairs which differ from the sequence of normal fibroblast ...

Detection, Cloning, Molecular Characterization and Phylogenic Analyses of a New Primate T-Cell Lymphotropic Virus Type I in Olive Baboon

THE IN VITRO GROWTH PROPERTIES OF CELL LINES FROM EPSTEIN-BARR VIRUS-INDUCED TAMARIN TUMORS AND TAMARIN B CELLS TR ANSFORMED BY EPSTEIN BARR VIRUS

EBV-carrying human cell lines, depending on whether the cells are derived from Burkitt's lymphoma (BL) tumor biopsies or transformed by EBV in vitro, have different growth properties in vitro. In contrast, there are no clear differences between tamarin tumor lines and tamarin LCLs in vitro. Both types of tamarin cell lines could grow in agarose and formed colonies unlike human LCLs, althoug...

Collagen binding site in collagenase can be determined using the concept of sense-antisense peptide interactions.

Tissue degradation and invasion are hallmarks of the metastatic phenotype. While several extracellular matrix components can be digested by proteases, degradation of interstitial collagen is selectively initiated by collagenase. It is obvious that inhibitors of collagenase activity would be extremely useful in preventing tissue destruction and tumor cell invasion and thus prove invaluable thera...